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In Chapter 14 of JoJo Part 1: Ghost Juice, DIO holds up a bottle of BBQ sauce exclaiming “I reject my humanity! Jojo!” He then downs the entire bottle within 3 panels (4 if you count the wide shot of everyone’s reactions) and then lets out a relieved sigh, only to begin coughing like a mad man, fall onto his knees and hands, throw up a bit of blood, and then he dies. However, for the story to progress, it is revealed that the Stone Mask in Dio’s shirt went off due to the coughed See More▼ up blood and struck him in the heart, turning him into the Hyper Vampire Queen. From there, Jojo and the gang must join forces and master Hamon to defeat Dio.